Bromothymol Blue Indicator | High-Purity Reagent Grade

Price range: $19.99 through $60.00

Chemical Information

  • Chemical Name: Bromothymol Blue (BTB)
  • CAS Number: 76-59-5
  • Molecular Formula: C₂₇H₂₈Brâ‚‚Oâ‚…S
  • Molecular Weight: 624.38 g/mol
  • Appearance: Yellow-green crystalline powder or dark blue solution (in water/ethanol)
  • Odor: Odorless

Physical & Chemical Properties

  • pH Transition Range: 6.0 (yellow) – 7.6 (blue)
  • Solubility: Soluble in ethanol, slightly soluble in water
  • Melting Point: 202–205°C (decomposes)
  • Absorption Maxima: ~427 nm (acidic form), ~615 nm (basic form)
  • Stability: Stable under normal laboratory conditions

Mechanical & Stability Properties

  • Light Sensitivity: May degrade upon prolonged UV exposure
  • Combustibility: Non-flammable
  • Storage Stability: Long shelf life under proper storage conditions

Description

Bromothymol Blue (BTB) is a high-purity pH indicator reagent widely used in laboratory analysis, scientific research, and industrial applications. It is commonly employed in acid-base titrations, medical diagnostics, water quality testing, and educational experiments. With its distinct color transition from yellow to blue across a pH range of 6.0 to 7.6, Bromothymol Blue provides precise pH measurement and reliable analytical performance.

Applications for Bromothymol Blue:

1. Laboratory & Analytical Chemistry

  • pH Indicator: Used in titrations and acid-base analysis.
  • Buffer Solutions: Employed in pH calibration and testing.
  • Spectrophotometric Analysis: Utilized in colorimetric pH measurement.

2. Medical & Biological Research

  • Respiratory Studies: Monitors COâ‚‚ levels in medical and physiological applications.
  • Cell Culture & Microbiology: Used to assess pH changes in biological media.
  • Diagnostic Kits: Component in blood gas and urinalysis test kits.

3. Environmental & Water Quality Testing

  • Aquatic pH Monitoring: Determines water alkalinity and acidity in environmental studies.
  • Aquarium & Hydroponic Testing: Ensures pH stability for aquatic life and plant growth.
  • Industrial Wastewater Analysis: Detects acidity fluctuations in effluent discharge.

4. Educational & Demonstration Experiments

  • Acid-Base Experiments: Used in high school and college chemistry labs.
  • Color Change Demonstrations: Engages students in hands-on learning of chemical reactions.
  • DIY Science Kits: Included in at-home chemistry and learning kits.

5. Industrial & Specialty Applications

  • Fermentation Monitoring: Used in brewing, biotechnology, and biofuel production.
  • Coatings & Dye Formulations: Incorporated into pH-sensitive inks and textiles.
  • Food & Beverage Testing: Evaluates acidity levels in food processing.

Storage

  • Store in a cool, dry, and dark environment.
  • Keep in tightly sealed containers to prevent moisture absorption.
  • Avoid prolonged exposure to light to maintain stability.

Handling Instructions:

  • Personal Protective Equipment (PPE):
    • Wear protective gloves, safety goggles, and a face mask when handling to avoid irritation.
    • Avoid direct skin or eye contact as the product is highly concentrated and pungent.
  • Ventilation:
    • Handle in a well-ventilated area or under an appropriate fume hood to avoid inhaling fumes.
  • Spill Management:
    • In case of spills, use absorbent materials like sand or cloth to clean up immediately.
    • Avoid water, as it can spread the product and worsen the spill area.
  • First Aid:
    • Skin Contact: Wash immediately with plenty of soap and cool water. Avoid hot water, as it may intensify irritation.
    • Eye Contact: Rinse eyes thoroughly with clean water for at least 15 minutes and seek medical attention.
    • Inhalation: Move to fresh air immediately. Seek medical attention if irritation persists.
  • Warnings:
    • Do not ingest.
    • Keep away from children and pets.

Safety

  • Hazard Classification: Non-toxic but may cause mild irritation upon contact.
  • Inhalation Risk: Avoid inhaling fine powder or vaporized solutions.
  • First Aid: If in contact with skin or eyes, rinse with water. If ingested, seek medical attention.

Additional information

Size

10 GRAMS, 25 GRAMS, 50 GRAMS

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